FastQCFastQC Report
Mon 3 Dec 2018
G166_M1.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M1.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9213534
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG1669341.8118346337029851Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG643810.6987655333990193No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA607720.6595948959432938No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC370620.40225607242562955No Hit
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG315990.3429628631098555No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC314500.34134567691398326No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT298090.32353492156212804TruSeq Adapter, Index 1 (100% over 50bp)
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC294240.31935628608957217No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC282880.3070265980458747No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG282520.30663586849519414No Hit
GGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCG233810.25376798956838925No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA230740.2504359347889745No Hit
GGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGAC209290.22715496572759158No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC197710.21458649851403383No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC161590.1753833002624183No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC158370.17188844150355337No Hit
CCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCCTCGTCGA157030.1704340592871313No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC157010.17041235208987127TruSeq Adapter, Index 1 (100% over 50bp)
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC152760.16579957267211476No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA151360.16428006886391258No Hit
GGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCT147610.1602099693776568No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG146530.15903778072561517No Hit
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC142740.15492426684483934No Hit
GGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGCAGGGGACCGAC142400.1545552444914188No Hit
GGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCC136620.14828186448326994No Hit
CGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGG134470.14594834077781665No Hit
GGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGG134240.14569870800932627No Hit
GGGTTCCCACGGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCG130140.14124873257101997No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG128010.1389369160628267Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAG127870.1387849656820065No Hit
CCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACC127710.13861130810392625No Hit
GCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGG127360.13823143215187572No Hit
GGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGG125850.1365925387587434No Hit
CCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCG120900.13122000743688578No Hit
GCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCC118670.12879965494239234No Hit
GCGAGGCCAGAAAAGCGTGGCATCGGGACGGGCGAACCCACACGCGACAC117550.12758405189583064No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT106000.11504814547816289No Hit
TCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACT105090.11406046800283148No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC102000.11070670602615675No Hit
GGCGACCGACCGGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGC101780.1104679268562964No Hit
GGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGG97220.10551868588100938No Hit
GGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCGAC93730.10173077995913403No Hit
ACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCG93530.10151370798653371No Hit
GACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGAC93440.10141602559886359No Hit
GCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCC92330.10021127615093188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT132750.040.27078244
TATGCCG137950.040.0764443
GATCGGA278950.038.561391
TACACGA231650.037.34216742
ATCGGAA295050.036.4787372
GTATGCC153050.036.35246342
TCGGAAG297700.036.1234783
CACGACG241900.036.01452344
TACACTC241550.035.6105529
CTACACT245850.035.0592928
AGTCTAC257400.034.51172625
CACTCTT247250.034.3625131
CAGTCTA261450.034.1622424
ACTCTTT248700.034.08255432
ACACTCT251750.033.9055830
TCTACAC260900.033.6440127
TTGCCGT14900.033.36879344
GTCTACA265650.033.24118426
CCAGTCT271700.033.06777623
CTACACG262500.033.02062241
Produced by FastQC (version 0.11.2)