FastQCFastQC Report
Mon 3 Dec 2018
G166_M1.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M1.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9213534
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115402712.525345866200743No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG1141221.2386343828546138Illumina Single End PCR Primer 1 (96% over 32bp)
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG376280.4083992092502182No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT346300.3758601205574321No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC285400.3097617049006386No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT268790.291733877576183No Hit
GGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGT216270.23473077757134234No Hit
TCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC154560.16775322042551752No Hit
GCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGG153040.16610347343375517No Hit
TCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTG152210.1652026247474639No Hit
TTCTCGGTCCCGTGCCCCGTGGGAACCCACCGCGCCCCCGTGGCGCCCGG139550.15146196888186442No Hit
GGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGTTGAGGGT127470.13835082173680588No Hit
GGCGGTCGACGAGGGCCGGTCGGTCGCCCTGCGGTGGTTGTCTGTGTGTG125900.13664680675189347No Hit
GGCGGTTGAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCC125350.1360498588272426No Hit
TTGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGC119030.1291903844930729No Hit
CCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGG116990.12697625037254978No Hit
TGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGCG115540.12540247857119755No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCTTTGCTCTC115440.1252939425848974No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGA114850.12465358026572648No Hit
TGACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGT107100.11624204132746457No Hit
TTGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGATGGCGC104350.11325730170421033No Hit
CGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCG100740.10933915259877482No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGG100720.10931744540151477No Hit
TGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGATGGCGCG98520.10692965370291138No Hit
GCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCCCCAACCCGGGTCG96530.10476978757553834No Hit
CGGGATTTGTGCCGGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGA95270.1034022341481564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG173850.038.14009529
GTAGACT179350.037.69418728
GACTGGA178400.036.9332531
AGACTGG181650.036.5023730
ACTGGAG186300.035.52081332
TGTAGAC221050.035.42017427
CTGGAGT185750.035.31958433
GGAGTTC216150.035.2683235
TGGAGTT195700.034.76045234
AGTGTAG262300.032.60927625
GTGTAGA265100.032.31464826
ATCGACA12800.031.7968715
GATCGGA94500.031.6862011
ATCATCG12650.031.6537152
GAGTGTA276750.031.42334224
TAGACGG7250.030.95104829
AGAGTGT284900.030.85646623
ATCGGAA98450.030.6383632
TCGTGTA286400.030.58831811
GTAGACG7700.030.57076328
Produced by FastQC (version 0.11.2)