FastQCFastQC Report
Mon 3 Dec 2018
G166_M10.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M10.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23698983
Sequences flagged as poor quality0
Sequence length50
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG3671541.5492394758036663No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG2291820.9670541558682075Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG2004770.8459308148370756No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1931100.8148450927198017No Hit
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG1772170.7477831432682154No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC1675520.7070008025238889No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC1522510.6424368505602118No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1520420.6415549561768115No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC1374000.5797717142545737No Hit
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC1294400.5461837750590395No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC1288710.543782828149208No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA1234820.5210434557466032No Hit
GGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCG1213390.5120008736239863No Hit
GGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGAC1052840.4442553505355061No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG878450.3706699144009682No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGGT848230.3579183123596485TruSeq Adapter, Index 15 (97% over 40bp)
GGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAG813680.3433396276962602No Hit
GGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACACC795840.3358118785097234No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTT794710.33533506480003805TruSeq Adapter, Index 15 (97% over 40bp)
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA775090.32705622853098804No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC770630.3251742912343538No Hit
CGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGG769120.3245371330913229No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC708640.29901705064727885No Hit
GGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGCAGGGGACCGAC650710.27457296374279017No Hit
GGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCT648970.27383875502168176No Hit
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA642170.27096943358286724No Hit
GGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGACAGGACC627260.2646780243692314No Hit
GCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGG627180.2646442676464218No Hit
GCGAGCGAAGCCGTCGGGGGTGGGACAGGACCAGGCGGGACAGAGAGCGC617690.2606398764031351No Hit
GGGTTCCCACGGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCG607150.25619242817297266No Hit
GGGAACGACACGGGAACCCACGACCGGCACCGACAGGACCGACCCGCGGA594860.2510065516313506No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC580620.244997854971245No Hit
CCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCCTCGTCGA568800.24001029917612918No Hit
CCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACC567980.23966429276733098No Hit
GGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCC559920.23626330294426562No Hit
GGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCGGC525800.22186606066597878No Hit
GGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCGAC513810.21680677183489264No Hit
GGGAACCCACGACCGGCACCGACAGGACCGACCCGCGGAAATCGGGCAAG503250.2123508844240278No Hit
GGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGG500400.21114830117393646No Hit
GGGGGACGGCGGGGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCG490740.2070721768946794No Hit
GGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGCGCT486040.20508896942961646No Hit
CGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGA480860.202903221627696No Hit
GGGCGCTCGCGCGGAACGGGAGAGTGCATGCGGCCGGACCGACCCCGTCG471180.19881865816773658No Hit
GGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGA463010.195371252850808No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTTGGC460700.1943965274796813TruSeq Adapter, Index 15 (97% over 37bp)
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC458170.19332897112082828No Hit
CGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAG444860.18771269636338403No Hit
GGCGACCGACCGGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGC442720.18680970402822772No Hit
CCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCG439950.18564087750094593No Hit
GCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAG439200.18532440822460608No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGGTGGC436850.18433280449207462TruSeq Adapter, Index 15 (97% over 37bp)
GGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGC434110.18317663673584642No Hit
GCGAGGCCAGAAAAGCGTGGCATCGGGACGGGCGAACCCACACGCGACAC433080.18274201892967307No Hit
ACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCG431160.1819318575822431No Hit
CGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAG425950.17973345100926905No Hit
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC422990.1784844522653145No Hit
CGGCACCGACAGGACCGACCCGCGGAAATCGGGCAAGCCGGCGGCGCGGG411210.17351377483160352No Hit
GGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGG409910.17296522808594783No Hit
CGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCC409780.17291037341138224No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT405060.1709187267656169No Hit
GCGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCT398430.1681211383627728No Hit
GGACGGACGCGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACC396820.16744178431622994No Hit
GGGAGCGGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGAC396430.16727722029253322No Hit
CCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCT394450.16644174140299609No Hit
GCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCC375030.15824729694097No Hit
GGCGGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGC364350.1537407744458908No Hit
GAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGACC363290.1532934978686638No Hit
CCGGGTGAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCC361650.15260148505106738No Hit
GGCGGAGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCGACACCCG356080.15025117322545023No Hit
GAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACG345770.1459007755733653No Hit
CCGCCTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGG338740.1429344035564733No Hit
GGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTCC337960.14260527550907987No Hit
GGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGG336920.1421664381125553No Hit
GCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCC336380.1419385802335906No Hit
GGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGT334180.1410102703563271No Hit
GCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAGCACA317920.13414921644527952No Hit
GGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGG317870.13412811849352355No Hit
CGGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTC316260.1334487644469807No Hit
CAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGC309700.13068071317659496No Hit
GGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGACCGCCCCTAGCGG304510.12849074578432332No Hit
GGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCG302550.12766370607548855No Hit
GCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCT298640.12601384624817022No Hit
CGAGAAGCACACAGCACGCACCACGCGCGGCGCAGGCCCGCGTCGCGGGA290050.12238921813649134No Hit
CGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCG287170.12117397611534639No Hit
GGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCACA282410.11916545110817625No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA280120.11819916491775195No Hit
CGCCTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGC278550.11753668923261391No Hit
GGCGCCGGAGCGGGTACCGCCCGGGTGAAGGAGAAGCGGAGACGAAGAAG274590.11586573145353958No Hit
GGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGA269890.11388252398847663No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCT269660.11378547341039909No Hit
CGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGA266880.1126124272927661No Hit
CTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCG266790.11257445097960533No Hit
GCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGG265250.11192463406552086No Hit
GGGGGACGGCGGAGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCG264720.11170099577690738No Hit
GCGGACGCGCCCACCCCCGGGCGCCACGGGGGCGCGGTGGGTTCCCACGG263290.1110975943566861No Hit
TCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACT259130.10934224477058783No Hit
CGGGACAGCTCTCCGACACCTCTCTTACCCGCTCTCCCCTCCGTCCCCGG257990.10886121147055129No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG256110.10806792848452611Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCGGGGGAGACCAGGCTCACGCGACGCGGGGCGCGCGCGCCACACCGACA255930.10799197585820454No Hit
GGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGC253810.10709742270375061No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGGTGGG252890.10670922039144042TruSeq Adapter, Index 15 (97% over 37bp)
GCGCGCGCCACACCGACAACCGCCCACACGTCTGAACTTCGGGAGACGGA252630.10659951104230929No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT250110.10553617427380745TruSeq Adapter, Index 15 (97% over 40bp)
GGGTGAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCC250030.10550241755099786No Hit
GGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAA245190.10346013582101814No Hit
GCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCG243910.10292002825606483No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG240810.10161195524719352No Hit
GGGGGAGACCAGGCTCACGCGACGCGGGGCGCGCGCGCCACACCGACAAC239640.1011182631761034No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA238980.10083977021292433No Hit
GCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACG238960.10083133103222193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA484750.038.7588161
CGGTTGG92300.038.4459243
TACACGA329300.036.32997542
TCGGTTG87000.036.2869342
ATCGGAA524300.035.9739382
TCGGAAG527050.035.7775843
CGGTTTG39950.034.6929643
TGATACG18900.034.4557153
TACGGCG18900.034.104776
TACACTC353050.033.91653429
AGTCTAC367800.033.48945625
CAGTCTA369750.033.47348424
ACTCTTT350450.033.37092632
CACTCTT350850.033.3454231
CTACACT362500.033.14160528
ACACTCT355350.033.07792330
TCGGTTT39050.033.013742
GTCTACA374000.032.68723326
CCAGTCT384350.032.34504723
ATACGGC20200.032.237525
Produced by FastQC (version 0.11.2)