FastQCFastQC Report
Mon 3 Dec 2018
G166_M10.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M10.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23698983
Sequences flagged as poor quality0
Sequence length50
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360247515.20096875043119No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG1492350.6297105660609994Illumina Single End PCR Primer 1 (96% over 32bp)
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG1098230.4634080711395928No Hit
GCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGG891710.37626509120665647No Hit
CCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGG876910.37002009748688375No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT812350.3427784221795509No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC779960.32911116903202137No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT767250.32374806969564895No Hit
CCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCG592460.2499938499470631No Hit
TCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTG532790.22481555432146602No Hit
TTGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGC529890.2235918731196187No Hit
GGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGT487850.20585271528318325No Hit
CGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGG476940.2012491422100265No Hit
GTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCCCCCGCG476830.20120272671616332No Hit
GGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGTTGAGGGT469460.19809288862733054No Hit
CCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGT448490.1892444076608688No Hit
TGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGCG440730.18597000554833934No Hit
GGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGG415910.17549698229666646No Hit
GACGAGGGCCGGTCGGTCGCCCTGCGGTGGTTGTCTGTGTGTGTTTGGGT406560.17155166531829658No Hit
CCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCG395340.16681728494425266No Hit
CGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCG378590.1597494711059964No Hit
GCGGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCC371050.1565678999811933No Hit
CGGGATTTGTGCCGGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGA367800.15519653311705403No Hit
GGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGC366660.15471549981701746No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCTTTGCTCTC365440.15420070979417133No Hit
GGCGGTTGAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCC356820.15056342291143884No Hit
CGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCC356470.15041573724914695No Hit
TCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC350410.1478586654963211No Hit
GGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGAGAGCTGTCCCGGG349690.14755485499103485No Hit
TGACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGT338560.14285845093015173No Hit
GGGGTTGTCGGGGTGGCGGTCGACGAGGGCCGGTCGGTCGCCCTGCGGTG334970.14134361799407175No Hit
GCCCGTGCACCCCGGTCCTGGCCTCGCGTCGGCGCCTCCCGGACCGCTGC333890.14088790223614236No Hit
GGCGGTCGACGAGGGCCGGTCGGTCGCCCTGCGGTGGTTGTCTGTGTGTG329160.1388920360000258No Hit
GCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCCCCAACCCGGGTCG320700.13532226256291252No Hit
GCCCTCGCCCGTGCACCCCGGTCCTGGCCTCGCGTCGGCGCCTCCCGGAC318960.13458805384180408No Hit
CCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCC315490.13312385598993848No Hit
GGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGC315220.13300992705045614No Hit
GGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGTGTGGC309740.13069759153799973No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGG302730.12773965870181012No Hit
GCCTGTCTGAGCGTCGCTTGACGATCAATCGCGTCACCCGCTGCGGTGGG297360.1254737386832169No Hit
GAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCCGCGGGGT295590.12472687119105491No Hit
GGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCG291750.12310654849619497No Hit
TTCTCGGTCCCGTGCCCCGTGGGAACCCACCGCGCCCCCGTGGCGCCCGG290290.12249048830492008No Hit
GGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGACGATCAATCG285300.1203849127196724No Hit
GGGTACCTAGCTGTCGCGTTCCGGCGCGGAGGTTTAAAGACCCCGGGGGG283320.11954943383013525No Hit
CGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCG277510.11709785183608933No Hit
GCAGGGCCAACCCCCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCA277310.11701346002906537No Hit
GCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCCCCAACCCGGGTCGGGC264750.11171365454796098No Hit
GCTCGCAGGGCCAACCCCCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAG262510.11076846630929267No Hit
GGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGT259830.10963761609517168No Hit
GCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCCCCCGCGCATCCGCGCTC259650.10956166346885012No Hit
GGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTC258830.1092156570600519No Hit
CGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCG251440.10609737979051675No Hit
GCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGA250710.10578934969487931No Hit
GCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAA248040.1046627190711095No Hit
CGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGG244700.10325337589380944No Hit
GCCAACCCACCGCCCTGTGCTCCGCGCCCGGTGCGGTCGACGTTCCGGCT239630.10111404358575217No Hit
GGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCCCC238350.10057393602079887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG237700.038.13133229
GTAGACT246850.037.5467428
GACTGGA241350.036.95321331
AGACTGG249200.036.195130
ACTGGAG249900.035.80335232
CTGGAGT249900.035.5941733
TGTAGAC297450.035.46407327
TGGAGTT267100.034.66117534
GGAGTTC296850.034.09276235
GCCGTAT17200.034.0236479
AGTGTAG348100.033.17292425
GTGTAGA352550.032.5794826
GAGTGTA365850.032.04452524
ATCGACA23200.031.4828555
CGTATCA18700.031.2944811
AGAGTGT379900.031.23002423
AAGAGTG382900.031.08875822
TCGTGTA386650.030.73719411
CGTGTAG388800.030.5106412
ATCATCG23700.030.4485052
Produced by FastQC (version 0.11.2)