FastQCFastQC Report
Mon 3 Dec 2018
G166_M11.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M11.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20817887
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG2314041.1115633397376015Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGGT657680.3159206311380209TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTT560220.26910512099522876TruSeq Adapter, Index 21 (97% over 40bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG529890.2545359190392377No Hit
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA508830.24441961857127958No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG481000.2310513069842295Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG414700.19920369439991675No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG396560.19049003388288158No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG378110.18162746296009774No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA329200.15813324378213794No Hit
GGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGC322540.15493407183927935No Hit
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG316470.15201831002349087No Hit
GCGGTAGACGAGAGAGCAAACGCTGCGCGCGCGGGAGGCGGTCAGGGGGC309700.14876629890439888No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC305680.14683526719114193No Hit
GGGAAAGCAGGAAGCGTGGCTCGGGGACAGCTTCAGGCACCGCGACAGAC288080.138380998993798No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC284040.13644036015758948No Hit
GGGCTTCTCAGACACAAACGGGAAGGCACACAGCCAGACGGAGCACCGGA273020.13114683541129799No Hit
GCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAG258010.12393668963617682No Hit
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC256940.12342270855827012No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC246180.11825407641034846No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA241380.11594836690198193No Hit
GGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCGGC241190.11585709923394243No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG231670.11128410870901548No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC224230.10771025897104736No Hit
GGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGAC209160.1004712918270716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA448150.040.812051
ATCGGAA481800.038.2257352
TCGGAAG489500.037.6372833
TACACGA337800.034.82378842
CACGACG366050.032.5689844
CGGTTGG41050.030.86987143
TCGGTTG36050.030.20823742
TACACTC392650.029.442329
AGTCTAC423800.029.23521625
CAGTCTA423350.028.92852424
TCTACAC415350.028.63827127
CTACACT409650.028.38158628
ACTCTTT418150.027.231232
GTCTACA448050.027.19136826
CCAGTCT455200.027.12672823
ACACGAC444500.027.05349243
CACTCTT427950.026.97259131
ACACTCT431800.026.87984530
CTACACG440550.026.83662841
TCCCTAC426200.026.69749638
Produced by FastQC (version 0.11.2)