FastQCFastQC Report
Mon 3 Dec 2018
G166_M11.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M11.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20817887
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221257210.628225621553234No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG1388080.6667727613277946Illumina Single End PCR Primer 1 (96% over 32bp)
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG244490.11744227452094441No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC221450.10637486888078505No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT217030.10425169470849756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA146100.036.7593541
ATCGGAA162250.034.1442952
GTAGACT246600.033.93555528
TAGACTG239350.033.71346729
TCGGAAG170150.032.9597553
GACTGGA255100.031.14094731
GGAGTTC307000.030.60794335
AGACTGG265900.030.28934930
AGTGTAG378500.029.94452925
GTGTAGA379950.029.76077326
GAGTGTA396750.029.17705324
TGGAGTT289500.029.02311734
ACTGGAG274300.028.96935532
TCGTGTA403450.028.7977811
CGTGTAG408950.028.5180712
TGTAGAC346300.028.44717427
CTGGAGT279850.028.1292433
AGAGCGT435550.028.0588745
CGTCGTG431700.027.9936879
AGAGTGT418750.027.83329823
Produced by FastQC (version 0.11.2)