FastQCFastQC Report
Mon 3 Dec 2018
G166_M12.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M12.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34011303
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG6894692.027176083197989Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT6380461.875982228613823TruSeq Adapter, Index 25 (97% over 44bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCC5473681.609370861210463TruSeq Adapter, Index 25 (97% over 41bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG2599010.7641606674110663No Hit
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG2011900.5915386423154678No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCGCGTAT1790840.5265426026165478TruSeq Adapter, Index 25 (97% over 45bp)
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA1775540.5220440981046801No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCGCGTATGCC1638720.4818162950122787TruSeq Adapter, Index 25 (97% over 42bp)
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG1459240.42904560286914034No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1356190.3987468518921489No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1255310.36908612410409564No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC1181230.34730512970937927No Hit
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC1095700.3221576074283305No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG1083720.31863524899354784Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC1074180.31583029912144206No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA1053780.30983229310561844No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC1045410.3073713465197143No Hit
GCATGCGTCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAG979770.2880718801040936No Hit
GGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGAC849630.24980812996197177No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC838710.24659743262408967No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG836220.24586532306627593No Hit
GGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCG781480.22977067359048253No Hit
GCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAG717530.21096810081048645No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA715230.21029185503419262No Hit
GCGGCAACAGGAAGGTTCTCTTCCAAGGGCATTCTGAGCATCCGCGGACA704020.20699589192451698No Hit
GGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCGGC673770.1981017898667393No Hit
GGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTCC670390.1971080025954901No Hit
GGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGC669920.19696981324120394No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC659220.19382380028192392No Hit
GGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCT657640.19335924883560032No Hit
GGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCACA623390.18328906716687685No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG606280.17825838663105617TruSeq Adapter, Index 25 (97% over 43bp)
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG598210.17588564601597298No Hit
GCGGTAGACGAGAGAGCAAACGCTGCGCGCGCGGGAGGCGGTCAGGGGGC592810.17429793854119613No Hit
GGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACACC577170.169699467262398No Hit
CGGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTC567270.16678867022530716No Hit
GCGAGCGAAGCCGTCGGGGGTGGGACAGGACCAGGCGGGACAGAGAGCGC553540.16275177696073567No Hit
GGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAGAC546970.16082006619975717No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC531550.1562862792995611No Hit
GCGGTCAGAAAGGGGGGACACGCGCCCAGCCGCGGGAAGAAGAGAGCGAG523400.153890017092259No Hit
GGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGCAGGGGACCGAC523330.15386943569906747No Hit
GCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGG510880.15020888790999865No Hit
CGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGG507150.14911219367279166No Hit
GGGTTCCCACGGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCG505670.14867704421674172No Hit
GCGAGGCCAGAAAAGCGTGGCATCGGGACGGGCGAACCCACACGCGACAC493640.1451399847868222No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC488880.1437404500497967No Hit
CCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACC483410.14213216118182828No Hit
CCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCCTCGTCGA482690.14192046685185805No Hit
GCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAGCACA476310.14004461987239947No Hit
GGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAG475960.13994171290644172No Hit
GGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCC473310.13916256016419012No Hit
CCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCG450740.1325265309594284No Hit
CCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCT438190.12883658118008592No Hit
GGCGAGCGGGGAGGGGGGAACGACACAGCAGAACGAGGGCCGGCGACGAC433940.127586996593456No Hit
GGGAACGACACGGGAACCCACGACCGGCACCGACAGGACCGACCCGCGGA423650.12456153179429791No Hit
GGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGG423450.12450272781375062No Hit
GCGGGAAGAAGAGAGCGAGGAGGCGGGCGGGAAAGAGACGGCACCCGGCG416230.12237990411599345No Hit
GGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCGAC412930.12140963843696315No Hit
GCGAAGCCGGCGAGAGAAGACCACGCCAACGGGGGTCGGGCAGGAGAGAC409620.12043643255890549No Hit
GGGCTTCTCAGACACAAACGGGAAGGCACACAGCCAGACGGAGCACCGGA398180.1170728448716005No Hit
GAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGACC397530.11688173193482179No Hit
GGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGC394220.11590852605676413No Hit
GGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGCGCT394190.11589970545968203No Hit
CGCGGTAGACGAGAGAGCAAACGCTGCGCGCGCGGGAGGCGGTCAGGGGG384250.1129771476264817No Hit
GGGAGCGGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGAC381420.11214507130173754No Hit
GGCGGAGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCGACACCCG379350.11153645010307307No Hit
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC374710.11017219775437595No Hit
GGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGACAGGACC369820.1087344404299947No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC366780.1078406199256759No Hit
CGGCTGCGGCGAGCGGGGAGGGGGGAACGACACAGCAGAACGAGGGCCGG363000.10672922469333208No Hit
CCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCAC362120.10647048717892403No Hit
CCAAACCACAGCTCCTCCCGCCCGCCGAGCCGACTTTCTTACCCCTCGGC358550.1054208361261549No Hit
CGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCC357440.10509447403411742No Hit
TGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACC348490.10246299590462617No Hit
GCAACGTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGA344970.10142804584699387No Hit
CGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGA343510.10099877678899863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC803600.041.1119343
GTATGCC810350.040.973144
GATCGGA1548550.039.838591
ATCGGAA1680150.037.0316122
TCGGAAG1686200.036.9267923
TCGTATG695250.036.75068742
TATGCCG42600.036.35696844
GCGTATG208500.036.21310842
TACACGA1008950.035.5093542
TACGGCG22300.034.429236
TACACTC1076650.033.3834429
AGTCAAC191750.033.32868228
GTCAACA191000.032.53807429
CAGTCTA1158350.032.48558424
CTACACT1119250.032.40966828
AGTCTAC1164150.032.38609325
TCTACAC1138300.032.23254827
GTCTACA1157500.032.13312526
ACTCTTT1113150.032.07145732
CACTCTT1115250.032.06033331
Produced by FastQC (version 0.11.2)