G166_M12.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G166_M12.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34011303
Sequences flagged as poor quality	0
Sequence length	50
%GC	69

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4494624	13.215089113169231	No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG	476919	1.4022367799316597	Illumina Single End PCR Primer 1 (96% over 32bp)
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG	107892	0.3172239534604129	No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT	99419	0.2923116471015533	No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT	75539	0.22209969432808851	No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC	66093	0.19432657431560327	No Hit
CGGGATTTGTGCCGGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGA	63290	0.18608519644190052	No Hit
CCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGG	59804	0.1758356626325078	No Hit
TCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTG	58679	0.1725279387267227	No Hit
GTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCCCCCGCG	54025	0.15884425245336822	No Hit
GGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGT	52425	0.15413993400958498	No Hit
GCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGG	47133	0.13858040075677194	No Hit
CCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCG	45246	0.13303224519213508	No Hit
TGACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGT	42636	0.1253583257307137	No Hit
GCCAACCCACCGCCCTGTGCTCCGCGCCCGGTGCGGTCGACGTTCCGGCT	40747	0.11980428976802213	No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCTTTGCTCTC	40640	0.11948968847209412	No Hit
TTGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGC	40035	0.11771086806053857	No Hit
GGCGGTTGAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCC	39699	0.11672296118734411	No Hit
GGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGAGAGCTGTCCCGGG	39655	0.11659359243014007	No Hit
GTGATTCCCGCCGGTTTTGCCTCGCGTGCCCTGACCGGTCCGACGCCCGA	38730	0.11387390832982788	No Hit
TCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC	37301	0.10967236391972399	No Hit
TGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGCG	36425	0.10709674957175266	No Hit
GCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCCCCAACCCGGGTCG	34679	0.1019631620699742	No Hit
GCCGCCCGAGTTCACGGTGGGTTCGTCCTCCGCCTCCGCTTCTCGCCGGG	34046	0.10010201608565247	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TAGACTG	72995	38.516483	29
AGACTGG	75050	37.265545	30
GTAGACT	76930	37.218365	28
GACTGGA	74780	37.059113	31
TGTAGAC	89390	36.275833	27
ACTGGAG	76635	36.190834	32
CTGGAGT	76660	36.058174	33
TGGAGTT	80850	35.4739	34
GGAGTTC	91825	34.22182	35
TAGACGG	2660	33.495174	29
GATCGGA	44045	33.138077	1
AGTGTAG	107985	32.79981	25
GTGTAGA	108975	32.465496	26
ATCGGAA	45800	32.218513	2
TCGGAAG	46730	32.019833	3
GAGTGTA	115735	31.207891	24
ATCATCG	3830	30.78993	2
AGAGTGT	120815	30.66227	23
TCATCGA	3880	30.506517	3
AAGAGTG	122115	30.472765	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content