FastQCFastQC Report
Mon 3 Dec 2018
G166_M2.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M2.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41191245
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1999730.4854745225593448No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT1885610.4577696061383918TruSeq Adapter, Index 2 (100% over 50bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG1668340.40502296058300735No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG1584040.384557446612745Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1527130.37074140390755367TruSeq Adapter, Index 2 (100% over 50bp)
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC943080.22895156482888537No Hit
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC904900.21968260488363484No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG903470.21933544373324965No Hit
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG817100.19836739579005197No Hit
GGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCACA757190.18382304297915733No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG751140.18235428426598904No Hit
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA745000.18086367624964964No Hit
GGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAGAC722650.17543776596216015No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC666120.16171397587035788No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA649410.15765728858159056No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGCCGT633820.15387250373228584TruSeq Adapter, Index 2 (98% over 50bp)
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC598340.1452590228821683No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC582220.14134556991418928No Hit
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC554460.13460627373608153No Hit
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG536160.1301635820912915No Hit
GACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGAC528470.1282966805203387No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC525350.1275392380104073No Hit
GGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGC495130.12020272754562286No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC463370.1124923512265774TruSeq Adapter, Index 2 (98% over 50bp)
GGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTCC440940.10704701933626916No Hit
GGGCTTCTCAGACACAAACGGGAAGGCACACAGCCAGACGGAGCACCGGA429200.10419689912261695No Hit
GCGAAGCCGGCGAGAGAAGACCACGCCAACGGGGGTCGGGCAGGAGAGAC427220.1037162144528528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG292650.039.9779343
ATGCCGT301950.038.75390644
GATCGGA429750.038.2065431
ATCGGAA457150.036.0415082
GTATGCC337400.034.7342642
TCGGAAG481900.034.1673283
TACACGA287200.028.31949242
TACACTC297350.028.24729329
AGTCTAC313500.028.0201625
CAGTCTA318200.027.27443124
CTACACT308400.027.27085728
TCTACAC329000.026.00464227
GTCTACA340550.025.27125726
ACTCTTT335450.024.59301832
CACTCTT347300.023.98826631
CTACACG344900.023.65833341
TATCGCG130100.023.13221236
CGCGTAT127900.023.11794539
TCGCGTA129500.022.9852138
CCAGTCT389950.022.5211223
Produced by FastQC (version 0.11.2)