FastQCFastQC Report
Mon 3 Dec 2018
G166_M2.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M2.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41191245
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG478477811.61600723648921No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG1065580.25869089414510293Illumina Single End PCR Primer 1 (96% over 32bp)
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC718810.17450552902685995No Hit
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG683920.16603528249753072No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT621490.1508791491978453No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC572630.1390174052762911No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT558000.1354656796608114No Hit
TCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC510250.12387341047836743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG197050.031.84066629
GATCGGA151750.031.635421
ATCGGAA154250.031.1937072
GTAGACT222200.028.97929228
TCGGAAG175350.027.8039383
TCGTGTA320800.026.6431311
AGTGTAG304100.026.58581425
CGTGTAG325750.026.2247612
GAGTGTA321750.025.64022324
GACTGGA245500.025.29736731
AGAGCGT365050.024.811465
TGGAGTT265450.024.3258934
AAGAGCG374250.024.3252164
AGACTGG259550.024.2751930
TGTAGGG357100.023.77461614
GTGTAGA349100.023.19663626
GTGTAGG368450.023.1915213
GTAGGGA372300.023.15812715
AAAGAGT370050.023.08426721
AGAGTGT366250.022.96337523
Produced by FastQC (version 0.11.2)