FastQCFastQC Report
Mon 3 Dec 2018
G166_M4.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M4.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20573978
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG10127364.922412184945468Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT7234633.5163982385905146TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2438521.1852447786227827TruSeq Adapter, Index 5 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGCCGT2107231.0242209843910595TruSeq Adapter, Index 5 (98% over 50bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT828030.4024647056587696Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC655240.3184799750442039TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC632110.30723761831571905TruSeq Adapter, Index 5 (98% over 50bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT616510.29965522467264233Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG541690.2632888982383475No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTAAGGTGGAA318940.15502106593095413No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC297550.14462443772419706TruSeq Adapter, Index 5 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGC272790.13258981807018555Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTAAGGTGGA265830.12920690398327442No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA232430.11297280477309737No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG211720.10290669116103848Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA70350.043.185298
TCGCCGT71100.042.853517
TACGGCG107400.042.56466
ACGGCGA110550.041.490977
TGGTCGC73900.041.440234
GCGACCA112200.040.90041410
ATACGGC113050.040.458645
ATGCCGT1095250.039.75801544
TGATACG115800.039.669983
TATGCCG1108200.039.46613343
CCGTATC78950.038.5089910
TGCCGTC106000.038.417344
CACGACG1299350.038.39937244
GATACGG119800.038.325964
GCCGTAT79450.038.2389569
GTGGTCG80200.038.1312033
CGTATCA79900.037.99606311
TACACGA1305450.037.938542
TACACTC1309250.037.91242629
CTACACT1332650.037.7832328
Produced by FastQC (version 0.11.2)