G166_M4.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G166_M4.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20573978
Sequences flagged as poor quality	0
Sequence length	50
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2493253	12.11847801139867	No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG	725298	3.525317272138621	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACAGGAGTTCAGACGTGTG	56804	0.2760963387829033	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT	40409	0.1964082978994145	Illumina Single End PCR Primer 1 (100% over 31bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT	34342	0.1669195913400899	Illumina Single End PCR Primer 1 (100% over 31bp)
TCCACCTTAGGGTAAAAATTCTCGTGTTCCGTTTGTACTCTAAGGTGGAA	32305	0.15701873502538014	No Hit
TTCCACCTTAGGGTAAAAATTCTCGTGTTCCGTTTGTACTCTAAGGTGGA	27913	0.13567138061487186	No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTT	24619	0.11966086480699066	Illumina Single End PCR Primer 1 (96% over 32bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TACGGCG	6960	42.451206	6
ACGGCGA	7210	40.978355	7
GCGACCA	7465	39.55034	10
TGATACG	8195	39.330345	3
TAGACTG	103890	38.84029	29
GACTGGA	103625	38.50294	31
GTAGACT	107260	38.442432	28
AGACTGG	104735	38.407482	30
CGCCGTA	10730	38.15699	8
CGACCAC	7720	38.01596	11
GCCGTAT	10730	37.972458	9
CCGTATC	10795	37.88648	10
CGTATCA	10810	37.793205	11
TCGCCGT	10835	37.786026	7
ACTGGAG	106490	37.434006	32
TGTAGAC	127020	37.10721	27
CTGGAGT	107170	37.049244	33
TGGTCGC	11235	36.853012	4
ACGTGTG	116090	36.763554	44
CCACCGA	8020	36.703648	14