FastQCFastQC Report
Mon 3 Dec 2018
G166_M5.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M5.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30653475
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG2281730.7443625885809032No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1755230.572603921741336No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1394710.4549924600718189TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG1009620.32936559394978876Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC957210.3122680218148187No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC879010.2867570479366532No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC795760.25959862625689256No Hit
GGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGG555090.18108550498760745No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT543440.17728495708887818TruSeq Adapter, Index 6 (100% over 50bp)
GACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGAC533090.17390850466382685No Hit
GGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGT529940.17288088870837645No Hit
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG525660.17148463591811366No Hit
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA498950.16277110507046919No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA458410.14954585083746624No Hit
GGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAA455500.14859652943100252No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC431060.14062353452585719No Hit
GGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGG430780.1405321908853727No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT416860.13599110704414427No Hit
GGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAGAC412550.13458506743525817No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG406960.13276145689844301No Hit
CAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCC392870.12816491441834899No Hit
GCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGG390430.12736891983698423No Hit
GGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCG381470.12444592334148086No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG361050.1177843621318627Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG359000.11711559619260134No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC357000.11646314161771218TruSeq Adapter, Index 6 (98% over 50bp)
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC344550.11240161188902727No Hit
GGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCG341010.11124676729147348No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC340930.11122066910847792No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC336320.10971676131335843No Hit
GGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCACA329000.10732877756926416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA448300.041.7934841
ATCGGAA471650.040.0043532
TCGGAAG487750.038.7597853
TATGCCG89850.036.97331243
ATGCCGT100650.032.98412744
TACACGA179650.029.39094742
CGTATGC281000.028.67091644
TATCTCG228800.028.61583539
AGTCAAC123800.028.60957328
GTATGCC118150.028.34069342
TCGTATG228800.028.33698543
CGCCAAT304100.028.28567333
CAATATC352300.028.24984736
TATCGCG62800.028.06095539
GTCACGC311500.027.94564629
AGTCACG317500.027.7224128
CCAATAT326800.027.5662735
TCACGCC316250.027.55373230
TCTCGTA238150.027.46463841
AATATCG70450.027.41698537
Produced by FastQC (version 0.11.2)