FastQCFastQC Report
Mon 3 Dec 2018
G166_M6.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M6.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805929
Sequences flagged as poor quality0
Sequence length50
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57797812.026353281540365No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC339970.7073970506014551No Hit
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG293530.6107664095745069No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT283410.5897090864222089No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT222750.46348999329786184No Hit
TCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC164030.3413075806987577No Hit
GGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCG145500.3027510393932162No Hit
CCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGG130490.2715187844015174No Hit
GAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCC127240.2647563041401569No Hit
TGACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGT123590.2571615186158597No Hit
GCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGG116470.24234648493558683No Hit
CGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCG116170.24172225598838434No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG109680.22821810309723678Illumina Single End PCR Primer 1 (96% over 32bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGG106860.22235035099353317No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGG103230.21479718073238288No Hit
GATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCT102420.21311176257493608No Hit
CGGGATTTGTGCCGGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGA101960.21215461152255893No Hit
GGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGT101260.2106980773124197No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGA100450.20901265915497297No Hit
TCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTG93230.19398954915896593No Hit
TTGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGC91780.1909724425808205No Hit
GCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCCCCAACCCGGGTCG89710.18666526284512316No Hit
TGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGCG88790.18475096074036884No Hit
GCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAA86990.18100558705715378No Hit
GTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCCCCCGCG84880.1766151767951628No Hit
GGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGC83650.17405583811163253No Hit
GGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC80530.16756385706072643No Hit
CCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG79430.16527501758765056No Hit
GCCCGTGCACCCCGGTCCTGGCCTCGCGTCGGCGCCTCCCGGACCGCTGC78970.16431786653527342No Hit
GCCCTCGCCCGTGCACCCCGGTCCTGGCCTCGCGTCGGCGCCTCCCGGAC73060.152020556275384No Hit
GGCGGTTGAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCC71010.14775499180283355No Hit
CCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCG70310.14629845759269436No Hit
CGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGG70290.14625684232954753No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCTTTGCTCTC70260.14619441943482728No Hit
GGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGACGATCAATCG69160.1439055799617514No Hit
GGCGGTCGACGAGGGCCGGTCGGTCGCCCTGCGGTGGTTGTCTGTGTGTG68790.14313569759353498No Hit
TTCTCGGTCCCGTGCCCCGTGGGAACCCACCGCGCCCCCGTGGCGCCCGG68650.14284439075150715No Hit
GGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGG67950.14138785654136796No Hit
GGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCC67570.14059716654157814No Hit
CCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGT67400.14024343680483004No Hit
GACGAGGGCCGGTCGGTCGCCCTGCGGTGGTTGTCTGTGTGTGTTTGGGT66720.13882851785783767No Hit
CGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGG65810.13693502338465674No Hit
GCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCCCCAACCCGGGTCGGGC64470.13414680075381888No Hit
GCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGG63420.1319619994386101No Hit
GGGGTTGTCGGGGTGGCGGTCGACGAGGGCCGGTCGGTCGCCCTGCGGTG62570.13019335075486965No Hit
GGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGC61850.12869520128158363No Hit
GGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC61730.12844550970270266No Hit
GGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGAGAGCTGTCCCGGG60660.126219093124347No Hit
GGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGTTGAGGGT60640.1261774778612002No Hit
GCTCGCAGGGCCAACCCCCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAG59640.1240967147038585No Hit
CCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCG59080.12293148733574716No Hit
CCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCG57960.12060103259952447No Hit
GCCTGTCTGAGCGTCGCTTGACGATCAATCGCGTCACCCGCTGCGGTGGG57780.12022649523120296No Hit
CGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCG57680.1200184189154688No Hit
GCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCC57060.11872834575791694No Hit
TTGCTCGCAGGGCCAACCCCCCAACCCGGGTCGGGCCCTCCGTCTCCCGA56670.11791684812655367No Hit
GAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCCGCGGGGT56540.11764634891609925No Hit
TCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAG56260.11706373523204358No Hit
GGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGTGTGGC56000.11652273681113474No Hit
CGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCC54190.11275655549634628No Hit
GGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTC54150.11267332497005261No Hit
GGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCAC51510.10718011023467056No Hit
GTTGGCCGAGGTGCGTCTGGGGGGCTCGTCCGGCCCTGTCGTCCGTCGGG50910.10593165234026554No Hit
CCGTGTCTACGAGGGGCGGTACGTCGTTACGGGTTTTTGACCCGTCCCGG50800.10570276839295795No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCCTTGCTCTC49740.10349715944617575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG19000.035.3145729
GTAGACT19550.034.99623528
ATCATCG13750.034.5606082
TCATCGA13850.034.311073
CATCGAC14400.033.9165534
ATCGACA14550.033.566555
GACTGGA20500.032.30132331
TGTAGAC24300.032.04827527
GATCATC15200.031.6979221
GATCGGA11200.031.232691
ATCGGAA11850.030.8191032
AGACTGG21850.030.7083230
AGTGTAG27450.030.37417825
CTGGAGT21650.030.18223633
TGGAGTT22500.030.11764734
ACTGGAG21700.030.00849232
TCGTGTA28700.029.58998311
GAGTGTA28850.028.97646924
CGTGTAG29850.028.52370512
GGAGTTC26550.028.42382435
Produced by FastQC (version 0.11.2)