FastQCFastQC Report
Mon 3 Dec 2018
G166_M7.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M7.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50577079
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG13691082.7069732516581277Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG1202220.23770055997104933No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGGCGG1083570.21424131670395594TruSeq Adapter, Index 8 (97% over 42bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGT846000.1672694463039275TruSeq Adapter, Index 8 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGCCGT789840.1561656022088583TruSeq Adapter, Index 8 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGGCGT681040.13465388145487803TruSeq Adapter, Index 8 (97% over 42bp)
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTAAGGTGGAA655520.12960811754273116No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGGCGG652970.12910393658755975TruSeq Adapter, Index 8 (97% over 46bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGGCGT652170.12894576217025108TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC620600.12270380422720734TruSeq Adapter, Index 8 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGCCGG612500.12110228825195697TruSeq Adapter, Index 8 (97% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGCCGG599510.11853393115090732TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGG598610.11835598493143504TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGG585680.11579949091168353TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC573820.11345455517508236TruSeq Adapter, Index 8 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGG530090.10480834608894675TruSeq Adapter, Index 8 (97% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGCCGT520720.10295572822621885TruSeq Adapter, Index 8 (97% over 42bp)
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG510530.10094098158574955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA39450.039.3143588
TTGCCGG208800.038.3733144
TTGCCGT192700.038.13155744
TACGGCG40250.037.7131776
TATGCCG221300.037.66716843
TACACTC1801550.037.44606429
GTTGCCG182500.037.30933843
TTTGCCG230100.037.3070143
CTACACT1823150.037.23650728
TACACGA1826200.037.22097842
TTTGGCG203950.037.20396843
AGTCTAC1881300.037.1063725
CACGACG1852000.037.06357644
ATGCCGG173750.036.94709444
CAGTCTA1905700.036.86907624
ATGCCGT197300.036.72960744
TCGCCGT42800.036.597027
TCTACAC1892450.036.1646827
CCAGTCT1972650.035.85865823
TATGGCG128750.035.6611243
Produced by FastQC (version 0.11.2)