FastQCFastQC Report
Mon 3 Dec 2018
G166_M7.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M7.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50577079
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG724879814.332179997978928No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG9868411.95116250189142Illumina Single End PCR Primer 1 (96% over 32bp)
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACAGGAGTTCAGACGTGTG724980.14334161132555717Illumina Single End PCR Primer 1 (96% over 32bp)
TCCACCTTAGGGTAAAAATTCTCGTGTTCCGTTTGTACTCTAAGGTGGAA664850.13145282668459363No Hit
TTCCACCTTAGGGTAAAAATTCTCGTGTTCCGTTTGTACTCTAAGGTGGA510610.1009567990274804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG1415450.039.0782229
GTAGACT1455900.038.76766628
TACGGCG39100.038.2607466
AGACTGG1454900.037.95664630
GACTGGA1458700.037.65453331
TGTAGAC1731550.036.82311227
ACTGGAG1520250.036.0925132
GGAGTTC1742100.035.69690335
CTGGAGT1530750.035.6827933
ACGTGTG1678000.034.78882644
AGACGTG1757000.034.74091742
GACGTGT1747050.034.71589343
TGGAGTT1638600.034.5694934
ACGGCGA44150.034.1330837
TAGACGG47850.033.83826429
CGAGCGT44100.033.524035
CAGACGT1793750.033.48861741
AGTGTAG2073850.033.4799825
TCAGACG1860100.033.4415440
GTGTAGA2072550.033.39589726
Produced by FastQC (version 0.11.2)