FastQCFastQC Report
Mon 3 Dec 2018
G166_M8.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M8.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34923694
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG4150371.188410939575865Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3740431.0710293132221351TruSeq Adapter, Index 9 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT3570701.0224290706475667TruSeq Adapter, Index 9 (100% over 50bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG3521371.0083039898356687No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA2251050.6445623993842118No Hit
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG1948220.5578504954258275No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC1715350.4911708366245564No Hit
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG1542780.4417573925599051No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC1483350.42474029236426136No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1396100.39975725362843917No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC1320800.3781959605991279No Hit
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC1252200.3585531358738855No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA1217110.34850551605451585No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC1081600.309703778758341No Hit
GGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCG1036730.2968557678921365No Hit
GGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGAC984250.2818287206387732No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGCCGT954950.2734390010403825TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC910530.26071984252295877TruSeq Adapter, Index 9 (98% over 50bp)
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC828690.237285895358034No Hit
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA810270.23201153921460885No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG809620.2318254191552589No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG795230.2277050073798035Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC787560.22550879067947394No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA772710.22125666316970935No Hit
GGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACACC693680.19862732733828214No Hit
GGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCT686140.19646833464982255No Hit
CGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGG678270.19421485023892376No Hit
GCGAGCGAAGCCGTCGGGGGTGGGACAGGACCAGGCGGGACAGAGAGCGC666560.19086182578509592No Hit
GGGTTCCCACGGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCG645490.18482867247662862No Hit
GGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCGGC642500.1839725202036188No Hit
GGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAG633940.18152146219125617No Hit
GGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGCAGGGGACCGAC632360.18106904727775933No Hit
GCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGG613020.17553125966571576No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC612230.17530505220896736No Hit
GGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCC600380.17191194035774107No Hit
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC579400.1659045575190299No Hit
CCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACC565330.16187577408048529No Hit
CCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCCTCGTCGA563990.16149208041967153No Hit
GGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGG545520.15620340734860408No Hit
GCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAG543460.1556135499297411No Hit
GGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGG530420.15187969520062797No Hit
GGGCGCTCGCGCGGAACGGGAGAGTGCATGCGGCCGGACCGACCCCGTCG526290.1506971169773736No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT522660.14965770803054224No Hit
GGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGCGCT496430.14214704778938908No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC482770.13823566315751135No Hit
GCGAGGCCAGAAAAGCGTGGCATCGGGACGGGCGAACCCACACGCGACAC477120.1366178503339309No Hit
GGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGACAGGACC476270.1363744625640117No Hit
GGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCGAC474260.13579892207279104No Hit
GGCGACCGACCGGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGC455210.13034417264107284No Hit
GGGAACGACACGGGAACCCACGACCGGCACCGACAGGACCGACCCGCGGA452910.1296855939695268No Hit
CCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCG452770.12964550657212837No Hit
GCGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCT447800.12822240396448326No Hit
GAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGACC446700.12790743155635254No Hit
GGACGGACGCGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACC444830.12737197846253032No Hit
GGGAACCCACGACCGGCACCGACAGGACCGACCCGCGGAAATCGGGCAAG440830.12622662425114595No Hit
GGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCACA431730.12362094342024645No Hit
GGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGA424530.12155930583975454No Hit
GGGGGACGGCGGGGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCG415570.11899371240625348No Hit
CGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGA411940.11795430345942212No Hit
GCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCC411000.11768514521974681No Hit
CGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCC408940.11709528780088384No Hit
GGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTCC406680.11644816267145164No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC399120.11428344321193515TruSeq Adapter, Index 9 (100% over 50bp)
GGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGC397090.11370217594965756No Hit
CGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAG396670.1135819137574622No Hit
ACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCG385180.11029188378526053No Hit
GCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCC384270.11003131570217056No Hit
TCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACT380900.10906635477907921No Hit
GGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCGG379230.10858816939582623No Hit
GGCGGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGC367200.10514351660508765No Hit
GGCGGAGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCGACACCCG357310.10231162831743973No Hit
CCGGGTGAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCC351140.1005449194463793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT524000.040.94352744
TATGCCG532350.040.81789843
GATCGGA991600.040.707551
ATCGGAA1079900.037.576922
TCGGAAG1090100.037.2487873
GTATGCC605650.036.03039642
TACACGA626200.035.04121442
TACACTC683150.031.53285429
CACGACG712900.031.08207144
CTACACT699350.031.02575728
AGTCAAC186250.030.21427728
AGTCTAC771700.030.0611825
CACTCTT709850.030.05547131
ACTCTTT713600.029.91294132
CAGTCTA776450.029.80927724
ACACTCT720800.029.78505930
TCTACAC771400.029.30286627
GTCTACA794200.029.02117326
CCAGTCT810850.028.62059623
TGCCGTC39300.028.60560844
Produced by FastQC (version 0.11.2)