FastQCFastQC Report
Mon 3 Dec 2018
G166_M8.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M8.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34923694
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG455709313.048714148050891No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG2772900.7939881731869486Illumina Single End PCR Primer 1 (96% over 32bp)
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG1364630.3907461793703724No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT1086090.31098943886061997No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT1063450.30450673402418427No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC1057090.30268562082808304No Hit
GCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGG940130.2691954636872033No Hit
CCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGG776910.22245928509166296No Hit
TCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTG640810.1834886080493089No Hit
TTGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGC633070.18127234765028005No Hit
CCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCG632770.18118644608442624No Hit
TGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGTTGGCGCG573940.1643411490204902No Hit
GGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGT538530.15420190086420985No Hit
GGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGTTGAGGGT516530.14790245270159566No Hit
GACGAGGGCCGGTCGGTCGCCCTGCGGTGGTTGTCTGTGTGTGTTTGGGT512990.14688881422452046No Hit
GTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCCCCCGCG508950.14573200647102222No Hit
TTCTCGGTCCCGTGCCCCGTGGGAACCCACCGCGCCCCCGTGGCGCCCGG478600.13704163139214312No Hit
GGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGG469350.13439299977831667No Hit
CGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGG466860.13368001678172992No Hit
TCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC459730.1316384228999372No Hit
CGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCG444070.1271543611623673No Hit
GGCGGTCGACGAGGGCCGGTCGGTCGCCCTGCGGTGGTTGTCTGTGTGTG443450.12697683125960274No Hit
CGGGATTTGTGCCGGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGA428380.12266170926821199No Hit
GGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGAGAGCTGTCCCGGG423820.12135600546723378No Hit
TGACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGT420450.12039104454414243No Hit
GGCGGTTGAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCC403720.1156006005550272No Hit
CCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGT397660.11386538892477983No Hit
GGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGC396170.11343874448103916No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCTTTGCTCTC386810.11075861562639966No Hit
CCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCG375390.10748862935289721No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGG371280.10631177790069973No Hit
GCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCCCCAACCCGGGTCG369790.10588513345695906No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCCTTGCTCTC367610.10526091541175454No Hit
CGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGGCCAACCCC362930.10392085098443482No Hit
GAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCCGCGGGGT361700.10356865456443409No Hit
GCCCGTGCACCCCGGTCCTGGCCTCGCGTCGGCGCCTCCCGGACCGCTGC359470.1029301195915873No Hit
GGGGTTGTCGGGGTGGCGGTCGACGAGGGCCGGTCGGTCGCCCTGCGGTG350910.1004790615792247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG430400.037.1800429
GTAGACT443800.036.82582528
GACTGGA440450.035.88256531
GATCGGA273700.035.7870941
AGACTGG455600.034.9932230
ATCGGAA288800.034.471542
ACTGGAG460150.034.45158832
CTGGAGT459600.034.28001433
TGTAGAC550100.033.86480727
TCGGAAG297650.033.816083
GGAGTTC537000.033.65335535
TGGAGTT490800.033.5129534
AGTGTAG664950.030.95020925
GTGTAGA671200.030.60961226
ATCATCG35150.029.7934552
ATCGACA35400.029.70625
CATCGAC35950.029.4966754
GAGTGTA707950.029.47123724
TCGTGTA733500.028.99789211
CGTGTAG739400.028.73972712
Produced by FastQC (version 0.11.2)