FastQCFastQC Report
Mon 3 Dec 2018
G166_M9.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG166_M9.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26286406
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACG2270830.8638799842017202Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2216200.8430973789265828TruSeq Adapter, Index 13 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCC1851000.7041662523206862TruSeq Adapter, Index 13 (97% over 37bp)
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG1397470.5316322056351105No Hit
GGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCG1355150.5155326292989616No Hit
GGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGA1296970.4933995160844735No Hit
CGGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAG1084860.41270761777018894No Hit
GGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGC1014060.3857735439375014No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG846390.32198772247525964No Hit
GGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCG828040.3150069279155165No Hit
GGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGC820730.3122260228347687No Hit
CGGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACAC785650.2988807218453523No Hit
GGGGTGCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGC743440.2828229922340848No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA731450.2782616992220237No Hit
GCATGCGTCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCGAG707340.2690896579775873No Hit
GGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGGA665870.25331344269733946No Hit
GGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGAC643620.24484899152816858No Hit
GCGGCAACAGGAAGGTTCTCTTCCAAGGGCATTCTGAGCATCCGCGGACA640850.2437952149107033No Hit
GGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAGAC630980.24004042241453624No Hit
GGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCACA623260.2371035431774127No Hit
CCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGCGC598190.2275662941521941No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA573970.21835240618287644No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCGCGTAT565710.21521009756906287TruSeq Adapter, Index 13 (97% over 40bp)
GGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGC544830.20726682833704996No Hit
GGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTCC525550.19993223873967406No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC512940.19513508236919114No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG509670.19389109336590177No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCGCGTATGCC497490.18925751964722753TruSeq Adapter, Index 13 (97% over 37bp)
CGGCGACGACGGCAAGGGCGGCCGAAACGGGCCCCTCCCGGAAGGGGGTC483330.18387070488069004No Hit
GCGAAGCCGGCGAGAGAAGACCACGCCAACGGGGGTCGGGCAGGAGAGAC477120.18150826704875514No Hit
GGGTTCCCACGGGGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCG474890.18065991980797982No Hit
GGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAG472400.179712662126576No Hit
GGCGGAGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCGACACCCG469230.17850671560045142No Hit
GCGAGCGAAGCCGTCGGGGGTGGGACAGGACCAGGCGGGACAGAGAGCGC461820.1756877680425388No Hit
GCGGTAGACGAGAGAGCAAACGCTGCGCGCGCGGGAGGCGGTCAGGGGGC454390.1728612119891932No Hit
GGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCT445460.1694640187783754No Hit
CGGGAGGCGCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGG429900.1635446093315305No Hit
GGACGGACACGGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACC428570.16303864438523852No Hit
GGCCCTCGTCGACCGCCACCCCGACAACCCCGCGGCCGCAGGGGACCGAC422410.16069522779188605No Hit
GGGACGCAACGGGTCAGCGAGGCCCTCTCCCTCCCCGAACTCGCAACACC413230.15720292838815622No Hit
GCGGTCAGAAAGGGGGGACACGCGCCCAGCCGCGGGAAGAAGAGAGCGAG411430.1565181637991896No Hit
CTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCA408690.1554757999248737No Hit
GGGACGCCAAACCCTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCC398630.15164872672209354No Hit
GCCGACGCGAGGCCAGGACCGGGGTGCACGGGCGAGGGCGGGAACGGCGG391600.1489743405774072No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC384560.1462961501850044No Hit
CGGCTGCGGCGAGCGGGGAGGGGGGAACGACACAGCAGAACGAGGGCCGG382020.14532987126501812No Hit
CCAAACCACAGCTCCTCCCGCCCGCCGAGCCGACTTTCTTACCCCTCGGC381780.14523856931982257No Hit
GGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGCGGCGGC380810.1448695572913239No Hit
CCAAACACACACAGACAACCACCGCAGGGCGACCGACCGGCCCTCGTCGA364070.1385012466139342No Hit
GGCGGAGAGCCTCGCGGGAGGACAAACCGGGGGTGAGCCGGGCGGGCGCT358200.1362681532043597No Hit
CGCGAAGCCGGCGAGAGAAGACCACGCCAACGGGGGTCGGGCAGGAGAGA355590.13527524455035808No Hit
GGCGAGCGGGGAGGGGGGAACGACACAGCAGAACGAGGGCCGGCGACGAC355230.13513829163256477No Hit
CCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACC353030.13430135713493888No Hit
GGGCGCTCGCGCGGAACGGGAGAGTGCATGCGTCCGGACCGACCCCGTCG352240.13400082156533685No Hit
GGGAAAGCAATGAGTCTCGAGAAACTTTCCAAGGCCAGCCGCGACCGCTC350170.13321334228802523No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC342510.13029928853720055No Hit
ACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCG338450.12875476396430915No Hit
GCGAGGCCAGAAAAGCGTGGCATCGGGACGGGCGAACCCACACGCGACAC335690.1277047915945603No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG330880.12587494844293282Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCGAACCACTGAGAAAAGTGCGCGCGGGGCAGCGCTCGGGCCGGAGAGCC329470.12533854951490897No Hit
GGGGGACGGCGGAGGCGAGCCCTGGATAGGCCACCCGTCGGAGCCCGGCG326010.12402227980500644No Hit
CCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCT323090.1229114394717939No Hit
GCAACGTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGA315880.12016857686821089No Hit
GCGGGAAGAAGAGAGCGAGGAGGCGGGCGGGAAAGAGACGGCACCCGGCG313190.11914523423247741No Hit
CCGGGACAGCTCTCCGACACCTCTCTTACCCGCTCTCCCCTCCGTCCCCG305500.1162197677385033No Hit
CGCGGTAGACGAGAGAGCAAACGCTGCGCGCGCGGGAGGCGGTCAGGGGG300820.11443937980719007No Hit
GCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCCC299800.11405134654010897No Hit
GGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGAGGACGAACCCACC297560.11319919505161719No Hit
GGGGCAGCGCTCGGGCCGGAGAGCCGCACCCCTGCCTTCCACACACCACC297190.11305843788610737No Hit
CGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCTCC296850.11292909346374701No Hit
AGACATCGACCGGAGCCCCCACCGCCACACCGGGACACGCGCGCGGGGTT295860.11255247293981535No Hit
CACGGGCGGGCGGAGGCCGCAGCGGGCCCCCCCCAAGCACACAGCAGCGA290200.11039926873228695No Hit
GGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAGACCAGGCTCACGCGAC288900.10990471652914438No Hit
GAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGACC284420.10820041355216077No Hit
CCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCG284160.10810150311153224No Hit
CGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGAAGCCGGC283300.10777433780791487No Hit
CGGGACAGCTCTCCGACACCTCTCTTACCCGCTCTCCCCTCCGTCCCCGG282290.10739010878855024No Hit
GGCCAACAGCCGTTCTCCCACAGAACGGTCCCGTCCCCGAGAGCGAGATC278840.1060776433263642No Hit
GCAACGCCACCGGCCACATCCACCGACTCTGGGAACATGGTCAAGCGAGA278250.10585319271109181No Hit
GGGCTTCTCAGACACAAACGGGAAGGCACACAGCCAGACGGAGCACCGGA274270.10433910211993225No Hit
GGAAAAACACGGGAACGACACGGGAACCCACGACCGGCACCGACAGGACC271540.10330054249333286No Hit
GCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCACTGCTAG270180.10278316480389141No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC269100.10237230605051144No Hit
GGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCGGC265910.10115875102895389No Hit
TTAAACCTCCGCGCCGGAACGCGACAGCTAGGTACCCGGGACAGGAGCGG264640.10067561156896078No Hit
GGGCGGAGGCCGCAGCGGGCCCCCCCCAAGCACACAGCAGCGAGGCGCAA263760.10034083776991043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC264650.040.62486643
GTATGCC273050.039.45566644
GATCGGA582350.038.936831
TCGTATG240600.036.52026442
ATCGGAA635200.036.036242
TCGGAAG642550.035.6850473
TACGGCG14600.035.1085556
GCGTATG68250.034.8775642
TATGCCG17100.034.3507844
TACACGA356250.033.37815542
TACACTC357100.032.1271329
CTACACT367300.031.39067328
ATACGGC16350.031.2175735
AGTCTAC402600.031.03716325
CAGTCTA405550.030.78427324
ACTCTTT374150.030.0574832
CACTCTT375800.029.9723431
GTCTACA409800.029.91744626
TCTACAC405050.029.77404427
CCAGTCT423150.029.57145323
Produced by FastQC (version 0.11.2)