G166_M9.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G166_M9.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26286406
Sequences flagged as poor quality	0
Sequence length	50
%GC	68

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	3308554	12.586558999355027	No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTG	156395	0.5949653216190909	Illumina Single End PCR Primer 1 (96% over 32bp)
CGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGG	91587	0.3484196356093716	No Hit
GACGATCAATCGCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTT	69520	0.264471301249779	No Hit
GGGAACCCACCGCGCCCCCGTGGCGCCCGGGGGTGGGCGCGTCCGCATCT	62919	0.23935946207328612	No Hit
GCCAACCCACCGCCCTGTGCTCCGCGCCCGGTGCGGTCGACGTTCCGGCT	50396	0.19171886791979093	No Hit
TCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTG	48054	0.18280931976779177	No Hit
CCCAACCCGGGTCGGGCCCTCCGTCTCCCGAAGTTCAGACGTGTGGGCGG	45669	0.1737361889639839	No Hit
GCGTCACCCGCTGCGGTGGGTGCTGCGCGGCTGGGAGTTTGCTCGCAGGG	42402	0.16130771167423955	No Hit
GTCGGTGTGGCGCGCGCGCCCCGCGTCGCGTGAGCCTGGTCTCCCCCGCG	41486	0.15782302076594268	No Hit
CGGGATTTGTGCCGGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGA	40796	0.15519808984157057	No Hit
GGCGCCCGGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGT	40635	0.1545856059592171	No Hit
TGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGATGGCGCG	39135	0.14887923438449518	No Hit
TTGGGTCTTGCGCTGGGGGAGGCGGGGTCGACCGCTCGCGGGGATGGCGC	39018	0.14843413740166683	No Hit
TCCTGTTGCCGCAGACCCCCCCGCGCGGTCCCCCGCGTGTTGGTCTTCTG	36585	0.13917840270746787	No Hit
GCCGCCCGAGTTCACGGTGGGTTCGTCCTCCGCCTCCGCTTCTCGCCGGG	34206	0.1301280973899589	No Hit
GGGGACGGAGGGGAGAGCGGGTAAGAGAGGTGTCGGAGAGCTGTCCCGGG	33054	0.12574560402057244	No Hit
GGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGAC	32548	0.12382065467603293	No Hit
TTTGACCCGTCCCGGGGGCGTTCGGTCGTCGGGGCGCGCGCTTTGCTCTC	31531	0.11995173474837145	No Hit
GTGATTCCCGCCGGTTTTGCCTCGCGTGCCCTGACCGGTCCGACGCCCGA	31264	0.11893600060807095	No Hit
CCGCCTCCGCTTCTCGCCGGGGGCTGGCCGCTGTCCGGTCTCTCCTGCCC	31125	0.11840721017548006	No Hit
CCGTCTCCCGAAGTTCAGACGTGTGGGCGGTTGTCGGTGTGGCGCGCGCG	30680	0.11671431994164588	No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC	29674	0.1128872467388657	No Hit
GCCCACCCCGCGCACTAGTACGCCTGTGCGTAGGGCTGGTGCTGAGCGGT	29337	0.11160521525841152	No Hit
GGCGGTTGAGGGTGTGCGTGCGCCGAGGTGGTGGTCGGTCCCCTGCGGCC	27923	0.10622600898730697	No Hit
GGGGGTGGGCGCGTCCGCATCTGCTCTGGTCGAGGTTGGCGGTTGAGGGT	26705	0.10159243526863276	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TAGACTG	24740	37.30289	29
GACTGGA	25720	35.35986	31
AGACTGG	26190	35.06963	30
GTAGACT	27180	34.731224	28
TGTAGAC	30640	34.549988	27
ACTGGAG	26730	34.007385	32
CTGGAGT	26920	33.671795	33
TGGAGTT	28665	32.896152	34
AGTGTAG	36225	31.870996	25
GTGTAGA	36230	31.775518	26
GAGTGTA	37800	31.10757	24
AGAGTGT	40155	30.159761	23
GGAGTTC	33745	29.984644	35
GATCGGA	14760	29.961395	1
TCGTGTA	41025	29.92903	11
AAGAGTG	40840	29.810057	22
CGTGTAG	41155	29.781033	12
ATCGGAA	15280	29.214901	2
GCCGTAT	1505	29.090137	9
AAAGAGT	42745	28.8932	21

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content