FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M23_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M23_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19127124
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT41000.020.7171696
CCGATCT44250.019.0331448
CGATCTC55350.015.3472559
CGACGAT17850.015.2196661
ACCGATC74550.011.5868637
CGTCGAT31150.011.5548293
CTTGACG63350.011.2491787
GTACCTT118200.010.7184778
CGCCGTA163800.010.68064140-144
TTGCGCG11001.474109E-810.4704478
CAAACCG94100.010.3274574
CGGAATT70300.010.2355771
TCGCCGT185850.010.188215140-144
GAGTACC115800.010.0080536
CTTAGAT103450.010.0076061
CGACACT64950.09.9482141
TCGTCGA38250.09.7897392
CGGAAAT81200.09.5849841
AAACCGA93300.09.4126435
TCTCGGT423350.09.404387140-144
Produced by FastQC (version 0.11.2)