FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M24_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M24_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24017839
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAG253920.10572141815090026No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244940.1019825305682164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC34300.016.5889132
CTCGAAT111450.015.834441
CTCCGTT132250.015.6047991
CGAATGT91450.014.0978953
TAAACTC311100.014.0677739
TCCGATT119050.013.7940052
CTCGATA47200.012.9779081
CTCCTAT124750.012.918711
GTATCAT121000.012.9091045
ATCATGC130050.012.8410187
CTCGATT32950.012.8363041
CTCCGAT202900.012.8307961
AGCTTTA384050.012.826074
CCGATTA112650.012.5316943
TATCATG125250.012.241196
TCGGTTT110200.012.1567172
CTCCCAT283100.012.0809321
GCTTTAA448850.011.97956855
TTGCGTG58900.011.9765737
TTCGATT68800.011.9344222
Produced by FastQC (version 0.11.2)