FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M25_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M25_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29026014
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG149250.024.0676173
CACTAGC217200.016.5380614
CCCACTA241400.015.3026322
ACTAGCC240750.015.0991245
TAGCCTC267950.013.5395967
CCCCACT319950.013.1020921
CGACGAT27700.011.6616381
CGGAATT116600.011.3015652
CTTAGAT151100.011.1264771
ACCCGCC95650.010.9874466
GACCCGC96750.010.6392685
CTAGCCT348500.010.6373476
GTTTCGG97700.010.3888724
TAGATCC148350.09.5107633
CGCCGTA299350.09.437346140-144
TCCCGCA298150.09.4165629
GATCTCG818550.09.050452140-144
TCGCCGT340900.08.99669140-144
CGTATCA245850.08.937468140-144
TCTCGGT722650.08.934448140-144
Produced by FastQC (version 0.11.2)