FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M29_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M29_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27261787
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG143250.027.0353953
CACTAGC205600.018.9066664
ACTAGCC233800.016.7178215
CCCACTA237350.016.2622242
TAGCCTC251700.015.5004557
CCCCACT294000.014.4116881
CTAGCCT324300.012.0082226
GTTTCGG80700.011.5069564
CGGAATT107150.011.0890982
GCATATC88700.010.468746
CGACAAT50200.09.65442851
CGACGAT27250.09.4316551
CGCCGTA319000.09.411026140-144
TTTCGGA103250.09.1328355
TCGCCGT360450.09.0238695140-144
GATCTCG851550.08.958255140-144
CGTTGAT39500.08.9234031
GCCGTAT310500.08.917421140-144
CGTATCA268500.08.81079140-144
GTCCGAT41700.08.8048161
Produced by FastQC (version 0.11.2)