FastQCFastQC Report
Fri 30 Nov 2018
G168_G169_M30_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M30_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27619991
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG135700.023.6071363
CACTAGC205800.015.8107134
ACTAGCC221050.014.52368455
CCCACTA237250.013.6586772
TAGCCTC244150.013.1791677
CCCCACT297800.012.69663051
GTTTCGG79650.012.2916884
CGACGAT27900.011.0522581
CGATCAC43750.010.6954353
GCATATC89800.010.6613576
CGACAAT54400.010.5269281
CGGAATT120450.010.2830982
CTAGCCT310750.010.2156176
TTTCGGA101450.010.075555
CGCCGTA284800.09.828181140-144
TCGCCGT320550.09.329483140-144
CTTAGAT147550.09.1057931
GCCGTAT283700.08.973043140-144
CCGTATC257250.08.899356140-144
GATCTCG769800.08.888231140-144
Produced by FastQC (version 0.11.2)