FastQCFastQC Report
Fri 24 Jun 2016
G74A_M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG74A_M4.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22427628
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8850.013.0745911
ATCGGAA10100.011.78622152
CGGTTCA11900.010.71167812
ATGCCGA11900.09.85654525
AGCGGTT13250.09.49141410
AGACCGT13300.08.82336231
GAGCGGT13100.08.8217879
GCGGTTC15100.08.55365511
GACCGTG15600.07.849546432
ACCGTGG16650.07.558535633
CGAGACC15600.07.522666529
AATGCCG16100.07.495938324
TCGGAAG16650.07.1456143
AAGAGCG18050.07.06172477
TGCCGAG18200.07.00714826
GAGACCG17350.06.763911230
CCGAGAC18100.06.483624528
CCGTGGA17950.06.44293934
CGGAAGA21100.06.36356354
GCCGAGA22150.05.681356427
Produced by FastQC (version 0.11.2)