FastQCFastQC Report
Fri 24 Jun 2016
G74A_M5.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG74A_M5.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17134773
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9750.018.326941
ATGCCGA10850.017.07723425
ATCGGAA11200.016.7045632
GACCGAC11300.015.65240732
CGGTTCA12100.015.310019512
AGCGGTT12300.015.19871910
ACCGACC11750.015.19751433
GAGCGGT13450.014.0255989
GCGGTTC14200.013.883183511
AGACCGA12600.013.76752431
AATGCCG13800.013.6721924
CCGACCT14250.012.41222934
CCGAGAC15200.011.97230628
CGAGACC15050.011.63960729
TGCCGAG16750.011.47116226
AAGAGCG17400.011.0372287
GAGACCG16750.010.96595830
TCGGAAG18350.010.3741083
CGGAAGA18750.010.1527954
GCCGAGA18600.09.599627
Produced by FastQC (version 0.11.2)