FastQCFastQC Report
Fri 24 Jun 2016
G74A_M7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG74A_M7.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23584995
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10000.015.1451461
ATCGGAA12450.012.0214072
CGGTTCA12850.011.63912512
ATGCCGA12950.011.28875225
AGCGGTT14100.010.48632910
AATGCCG17050.08.9724224
GAGCGGT17800.08.8794549
AGACCGT14400.08.85791631
GCGGTTC17400.08.7905611
CGAGACC16350.08.6338829
AAGAGCG22250.07.79105427
TGCCGAG19700.07.768352526
ACCGTGT17950.07.674296433
GACCGTG16950.07.525308632
TCGGAAG20600.07.26062063
CCGAGAC19100.07.212691328
GAGACCG20250.07.13903630
AGAGCGG26600.06.64472258
GCCGAGA23750.06.2294927
CGGAAGA25950.05.89469864
Produced by FastQC (version 0.11.2)