FastQCFastQC Report
Fri 24 Jun 2016
G74A_M8.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG74A_M8.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences31430898
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8850.07.69111541
TTGACGA85500.06.3256132
CTTGACG87400.06.26927041
ATCGGAA10700.05.88111352
TGACGAC93600.05.82913353
GACGACT94650.05.6387634
ACGACTT97100.05.4963215
CGACTTG98400.05.4061596
Produced by FastQC (version 0.11.2)