FastQCFastQC Report
Fri 24 Jun 2016
G74A_M9.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG74A_M9.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences29564149
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11000.09.5914341
ATCGGAA12850.08.6028682
CGGTTCA13550.07.90198112
ATGCCGA13200.07.59820925
AGCGGTT15200.07.267665410
CCGCTTG15350.06.980062534
ACCGCTT13650.06.852372633
GCGGTTC16950.06.81811611
GAGCGGT16700.06.61491549
GACCGCT15400.06.405225332
AGACCGC14550.06.311867731
CGAGACC18350.05.56101529
AAGAGCG19450.05.33017257
TCGGAAG21600.05.2720923
AATGCCG19750.05.25013124
TGCCGAG20100.05.24517926
CCGAGAC19300.05.199165328
Produced by FastQC (version 0.11.2)