FastQCFastQC Report
Tue 12 Jul 2016
SRR352447_GM12878_DNASE_CHROMATIN_DUKE.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR352447_GM12878_DNASE_CHROMATIN_DUKE.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16682188
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG472500.012.9770111
TCTGCTT475600.012.71570814
CGTCTTC479550.012.7094869
CCGTCTT482450.012.677588
CGTATGC485250.012.6245122
GCCGTCT484900.012.6053537
ATGCCGT485900.012.5903565
TATGCCG486250.012.5769814
TGCCGTC486050.012.5764026
TTCTGCT485050.012.48816913
GTATGCC491200.012.4587773
GTCTTCT490700.012.45684110
CTTCTGC492700.012.28410712
TCTTCTG497950.012.19747411
CGCGTAA1102.4384826E-79.555572510
ACGCGTA1251.5439982E-68.4086019
ATCCGCG2804.0326995E-96.2425042
TCCGCGA3152.1827873E-106.214763
ACAGTCG4300.06.01603171
GTCGTAT16700.05.94495731
Produced by FastQC (version 0.11.2)