FastQCFastQC Report
Tue 12 Jul 2016
SRR352449_GM12878_DNASE_CHROMATIN_DUKE.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR352449_GM12878_DNASE_CHROMATIN_DUKE.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82378538
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTATGCCGTCTTCTGCTT31108133.7762420595519672No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3217400.013.792291
CGTATGC3289500.013.4793662
TGCCGTC3318800.013.3647726
ATGCCGT3318250.013.3644655
TATGCCG3318300.013.3644544
GCCGTCT3320350.013.3596117
CCGTCTT3325000.013.3506978
CGTCTTC3323950.013.3459319
GTATGCC3345900.013.2642093
GTCTTCT3405800.013.05378110
TCTGCTT3407700.013.0126614
TTCTGCT3412450.013.00437313
CTTCTGC3416050.012.98279712
TCTTCTG3443950.012.87943511
GTCGGAC10250.08.6954341
TCGGACT13900.06.1979032
CTGCTTG216800.05.523786514
CGGACTG18000.05.12904833
Produced by FastQC (version 0.11.2)