FastQCFastQC Report
Tue 12 Jul 2016
SRR769954_IMR90_DNASE_GENOMIC.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR769954_IMR90_DNASE_GENOMIC.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23435350
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT859360.3666939047208597Illumina Paired End PCR Primer 2 (96% over 25bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA324050.1382740176698876Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG118550.024.74741723
GCGGTTC50300.022.2870458
CGGTTCA152100.019.9697551
ATGCCGA151750.019.82456814
TGCCGAG153250.019.65007615
AATGCCG156700.019.48515513
AGAGCGG150900.019.40365628
GAGCGGT144700.019.30638529
AGCGGTT144050.019.236330
ATCGGAA155650.018.8678322
AAGAGCG155100.018.84900927
CGGAAGA157050.018.60609424
GATCGGA158600.018.58273921
CGAGATC168800.017.79646718
AGATCGG166000.017.68142520
CCGAGAT170650.017.62015717
GCCGAGA178000.017.14508816
GTTCAGC181400.016.8962973
GAGATCG176750.016.78472719
GGTTCAG185950.016.5917172
Produced by FastQC (version 0.11.2)