FastQCFastQC Report
Fri 27 May 2016
Waxman-TP17_CGATGT_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP17_CGATGT_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37247804
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC724290.194451731973246TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT177950.023.79414642-43
CGATCAC103600.023.6069473
TATGCCG179900.023.52127546-47
ACGATCA105850.022.7996522
CGTATGC191250.022.38258444-45
CGATGTA188650.022.32337234-35
TATCTCG167950.022.19270538-39
ATCTCGT201700.020.6926840-41
CACGATC125900.020.3289131
CACCGAT209300.018.58707830-31
ACCGATG235900.018.22667132-33
TCACCGA238550.017.89689430-31
TCGTATG219650.017.6784242-43
ATGCCGT223150.017.27460746-47
GCCGTCT223500.017.26818748-49
CGGAAGA452750.017.2324394
ATCGGAA448750.017.2010672
GATCGGA447500.017.1684551
CCGTCTT250600.017.16355350-51
TGCCGTC247350.017.09767348-49
Produced by FastQC (version 0.11.2)