FastQCFastQC Report
Fri 27 May 2016
Waxman-TP17_CGATGT_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP17_CGATGT_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37247804
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA101400.021.6889172
CGATCAC100300.021.6022853
CACGATC117050.019.9807261
CGCCGTA89000.017.65951346-47
GATCTCG150350.014.38043834-35
CCGTATC125700.013.07690648-49
CGTATCA86700.012.87191148-49
TCGGGGG128900.012.66104338-39
CCACGAT122650.012.2826531
GTCGCCG133850.011.81167244-45
GAGCGTC255550.011.6261579
AGAGCGT262100.011.5839678
AAGAGCG300450.011.5755217
CCGGGAT92500.011.4103461
GCCGTAT106150.011.30190846-47
CGCCGGA52300.011.247107546-47
AAGACGT197750.010.9803223
ATTGGAC265450.010.6671716
TAGATCT186300.010.55726832-33
ATCTCGG181450.010.30129134-35
Produced by FastQC (version 0.11.2)