FastQCFastQC Report
Fri 27 May 2016
Waxman-TP18_ACAGTG_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP18_ACAGTG_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39785793
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC483800.12160119568309222TruSeq Adapter, Index 5 (100% over 50bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT424540.10670643161492345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC129500.027.50283
ACGATCA135750.026.0327512
CACGATC154000.023.6902871
TATGCCG134550.020.82191846-47
CTCGTAT131850.020.72060442-43
CGTATGC144650.019.62644444-45
ATCTCGT162250.016.60897440-41
GATCTCG151200.016.06990438-39
CCACGAT141700.016.0259931
TCGTATG171900.014.7433242-43
TGCCGTC201050.014.24697548-49
GCCGTCT184350.013.84762348-49
ATGCCGT182300.013.8375946-47
TCACAAG276550.013.5176226
ATTGGAC348050.013.4793036
AAGACGT251750.013.1131423
GATCGGA391000.012.75830651
TCTCGTA190100.012.74470340-41
CGTCTGA223150.012.70182816-17
AGTGATC214850.012.59773836-37
Produced by FastQC (version 0.11.2)