FastQCFastQC Report
Fri 27 May 2016
Waxman-TP20_CAGATC_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP20_CAGATC_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27293627
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC393930.14433039624964464TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT103500.023.97086342-43
TATGCCG104500.023.67273146-47
CGTATGC111750.022.30515744-45
ATCTCGT118650.020.3809540-41
GATCGGA241150.017.6347891
ATCGGAA249750.017.0168442
CGGAAGA266850.016.1345714
CCGTCTT159050.015.96287850-51
ATGCCGT137100.015.48304546-47
TGCCGTC160600.015.47583248-49
GCCGTCT141100.015.34067648-49
ACGTCTG145900.014.65997714-15
TCGTATG149900.014.41032342-43
TCGGAAG302350.014.3631523
AGAGCAC322850.013.667218
TCTCGTA161700.013.05673640-41
CATCTCG163200.013.05061538-39
CGTCTGA189250.013.02341316-17
CACACGT201600.012.61618112-13
ACACGTC174450.012.5939512-13
Produced by FastQC (version 0.11.2)