FastQCFastQC Report
Fri 27 May 2016
Waxman-TP20_CAGATC_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP20_CAGATC_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27293627
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA50600.019.66601446-47
GATCTCG91250.014.75158134-35
CGTATCA52050.012.90915648-49
CCGTATC82300.012.65607248-49
GTCGCCG86500.012.44475644-45
GAGCGTC146100.012.1886939
AAGAGCG170900.011.9978957
AGAGCGT151700.011.8613458
TCGTGTA87000.011.83758414-15
CGATCAC50400.011.623943
GCCGTAT62850.011.24582846-47
ACGATCA52750.011.1060952
TAGATCT115700.010.89077632-33
CTCGAAT71400.010.8099381
CACGATC69550.010.0278421
TCGGGGG88800.010.02696638-39
CCGGAAT84000.09.9633771
GATCGGA226500.09.9560451
TCGCCGT61750.09.86474644-45
CTCGTTT63900.09.7502841
Produced by FastQC (version 0.11.2)