FastQCFastQC Report
Mon 29 Jun 2015
G85_M1_CGATGT_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M1_CGATGT_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17739788
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC369740.2084241367484211TruSeq Adapter, Index 2 (100% over 50bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT177770.10020976575368318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA114550.036.5800971
ATCGGAA124850.033.6239742
CTCGTAT75750.032.49423242-43
CGATGTA76400.032.43068734-35
TATGCCG78600.031.87916246-47
CGGAAGA138850.030.9024014
CGTATGC82900.030.53277844-45
TATCTCG67500.028.99354238-39
CGATCAC53200.028.8360823
ATCTCGT85050.028.39449740-41
ACGATCA54200.028.3050882
TCGGAAG157650.027.010683
CACGATC61100.026.25991
TCACCGA98050.026.19449630-31
ACCGATG99800.025.96807332-33
CACCGAT86350.024.49483130-31
AGAGCAC181150.024.1233128
TGCCGTC104200.024.05033548-49
CGTCTGA107600.023.93620916-17
CCGTCTT109650.023.11118550-51
Produced by FastQC (version 0.11.2)