FastQCFastQC Report
Mon 29 Jun 2015
G85_M1_CGATGT_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M1_CGATGT_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17739788
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA29100.031.31923946-47
ACGATCA52300.026.5855582
CGATCAC54500.026.1949843
CACGATC61450.024.2909621
TCGTGTA41500.021.34536614-15
GATCTCG52250.020.95825834-35
CCGTATC44600.020.79965848-49
GTCGCCG47450.020.18734244-45
GATCGGA86800.019.6611041
TATCGCG15350.019.08485422-23
AAGAGCG76350.017.9677187
GAGCGTC66250.017.7585899
AGAGCGT67900.017.4642668
CGTCGTG55450.016.9399712-13
ATCGGAA101100.016.8807282
AGCGTCG62900.016.15370610-11
ATATCGC19050.015.744184520-21
CGTATCA31750.015.01164248-49
CCACGAT61600.014.8712381
CGGAAGA112400.014.4391864
Produced by FastQC (version 0.11.2)