FastQCFastQC Report
Mon 29 Jun 2015
G85_M2_TGACCA_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M2_TGACCA_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26413803
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1116760.42279409746487473TruSeq Adapter, Index 4 (100% over 50bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT371630.14069537809455157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA307200.043.324181
ATCGGAA321900.041.3038442
CGGAAGA332800.040.4252974
TCGGAAG367850.036.472133
CTCGTAT216250.033.24460242-43
AGAGCAC413400.032.7917758
ATCTCGT228200.031.04535740-41
TATGCCG249100.030.55977446-47
GAGCACA461900.029.7611989
ACGATCA84750.029.0175932
CGTATGC264950.028.87965644-45
CGATCAC87300.028.5421123
CGTCTGA283650.028.01956416-17
AAGAGCA489500.027.7033067
CACACGT296700.026.99152212-13
CACGATC94500.026.8684581
AATCTCG227650.026.3726738-39
CACGTCT304850.026.17078814-15
TGCCGTC289900.026.04519748-49
CCGTCTT292150.025.87043850-51
Produced by FastQC (version 0.11.2)