FastQCFastQC Report
Mon 29 Jun 2015
G85_M2_TGACCA_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M2_TGACCA_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26413803
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT357730.13543297797746126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC87300.035.741569
AAGACGG101750.031.4431938
ACGATCA83450.029.7576222
CGATCAC85550.029.3533063
CACGATC91400.028.796711
CGCCGTA97700.028.72311246-47
ATACGAG49650.027.44092816-17
GATCGGA206100.026.5565171
ATCGGAA219350.024.9529632
CCGTATC116400.024.7478348-49
GATCTCG138700.024.20538534-35
TCGTGTA109100.024.12389614-15
CGGCATA56750.024.09013712-13
AAGAGCG186300.023.5381037
GAGCGTC150250.023.1191239
GTCGCCG124000.023.0060344-45
GAAGACG137450.023.0057727
AGAGCGT157900.022.617878
CGGAAGA228050.021.9825744
CGTCGTG135850.019.9556912-13
Produced by FastQC (version 0.11.2)