FastQCFastQC Report
Mon 29 Jun 2015
G85_M3_AGTCAA_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M3_AGTCAA_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20249167
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT851590.4205555714958546TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA195100.050.007991
ATCGGAA212650.045.7776372
CGGAAGA227250.043.0604484
TCGGAAG253550.038.6672483
AGAGCAC278150.035.6487438
TATGCCG159300.032.98042348-49
CTCGTAT147300.032.42631544-45
CGTATGC171350.030.82007246-47
GAGCACA326750.030.6308279
ATCTCGT155950.029.89751842-43
AAGAGCA359900.027.512517
CCGTCTT195250.027.21981852-53
AATCTCG156350.027.02623740-41
ACGTCTG181850.026.95943514-15
TGCCGTC199200.026.31813450-51
ATGCCGT182400.025.89777448-49
GCCGTCT185200.025.63378150-51
GCACACG194000.025.48532510-11
CGTCTGA215400.025.25283416-17
CACACGT222550.024.54639812-13
Produced by FastQC (version 0.11.2)