FastQCFastQC Report
Mon 29 Jun 2015
G85_M4_AGTTCC_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M4_AGTTCC_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27495225
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT425460.15473959569343404TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT111900.029.28745344-45
TATGCCG116350.029.0308248-49
GATCGGA163150.028.2202871
TTCCGTA123300.026.93765336-37
CGTATGC127000.026.2458246-47
CCGTATC124600.025.78009638-39
ATCTCGT143100.023.2593742-43
ATCGGAA204550.022.5682952
CGGAAGA218700.021.6386344
TATCTCG104100.020.76475340-41
TGCCGTC167400.020.20662350-51
GTTCCGT118350.019.06989534-35
CGTCTGA181300.018.96362516-17
TCGGAAG249000.018.856113
CGTATCT116150.018.6105138-39
TCCGTAT118700.018.60216536-37
CACACGT187700.018.37966512-13
CCGTCTT191750.017.6043152-53
AGAGCAC282600.017.1572848
AGATCGG127250.017.1041471
Produced by FastQC (version 0.11.2)