FastQCFastQC Report
Mon 29 Jun 2015
G85_M4_AGTTCC_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M4_AGTTCC_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27495225
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA55000.029.46390546-47
TCGTGTA76400.020.93736514-15
GATCTCG93300.019.88572934-35
CCGTATC89400.018.4646548-49
AGAGCGT118300.018.2780679
CGTATCA59250.018.24672150-51
AGATCGG127900.017.0517561
GATCGGA136850.016.0726072
AAGAGCG147800.014.9130618
TCTCGGT108500.014.89276436-37
GTCGCCG118100.014.82399744-45
CGTCGTG118950.014.52288512-13
GCCGTAT78050.014.11970648-49
TAGATCT126050.013.94437932-33
GTCGTGT77650.013.803411514-15
TCGCCGT78950.013.517014546-47
GTATCAT126800.012.890100550-51
AGCGTCG144600.012.55780210-11
ATCGGAA175350.012.5171723
TCGGTGG141150.012.48557638-39
Produced by FastQC (version 0.11.2)