FastQCFastQC Report
Mon 29 Jun 2015
G85_M5_CCGTCC_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M5_CCGTCC_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21036038
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56450.022.52585448-49
ACCCGTC57800.022.23816132-33
CTCGTAT53750.022.22698444-45
CGTATGC68000.018.83438946-47
ATCTCGT65650.018.3042242-43
CCCGATC66300.018.01959438-39
GTCCCGA76950.016.9754836-37
CGTCCCG54050.015.73922534-35
CCGTCCC88700.015.22476134-35
GATCGGA115400.014.4272761
TCACCCG97150.013.80494330-31
TGCCGTC94100.013.71200650-51
CGCGTAT3406.707445E-613.6781761
CCGTCTT99450.013.39519352-53
GATCTCG60200.013.12737340-41
CCGATCT60900.012.93831638-39
ATCGGAA131850.012.6929992
CGTCTGA104150.012.65489116-17
CGGAAGA143050.012.5115074
CACACGT113300.011.79733512-13
Produced by FastQC (version 0.11.2)