FastQCFastQC Report
Mon 29 Jun 2015
G85_M5_CCGTCC_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M5_CCGTCC_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21036038
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA26400.024.39470146-47
GATCTCG51650.014.53964934-35
TCGTGTA46800.014.357450514-15
GTCGCCG48600.014.35174444-45
CGTATCA32000.013.87723950-51
CCGTATC48200.013.7955448-49
TCGCCGT34850.012.5422346-47
AGAGCGT74300.012.3294769
GCCGTAT39650.011.55161548-49
CTCGAAT58250.010.6972191
AAGAGCG89500.010.5473248
CGATTAG33750.010.3338194
CCACTAG74400.010.06290153
GTCGTGT48600.09.75931314-15
CGTCGTG69650.09.74745512-13
CGCATAT10205.820766E-119.573731
CGCGTAA9351.1623342E-99.4493951
CTCGGAT79500.09.3586861
CCCGATT25500.09.3001951
CTGGAAT257000.09.1916321
Produced by FastQC (version 0.11.2)