FastQCFastQC Report
Mon 29 Jun 2015
G85_M7_ATGTCA_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M7_ATGTCA_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26523692
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT995640.3753776058023898TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA767430.28933754772902653TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG262200.035.2616541
TATGCCG292550.033.75520348-49
CTCGTAT285100.032.96898744-45
GATCGGA370850.032.7311481
ATCTCGT299000.031.60744942-43
CGTATGC315100.031.49824146-47
ATCGGAA393300.030.8744722
CGGAAGA408950.029.96474
CCGTCTT349200.028.5944252-53
TGCCGTC347000.028.49410450-51
CACACGT382250.027.93773812-13
CGTCTGA386950.027.58581216-17
TCGGAAG450850.027.1385153
CACGTCT401550.026.58339314-15
AGAGCAC475850.025.8403118
ACATGTC413700.025.80550832-33
TCTGAAC449400.024.01371418-19
TCACATG447700.023.97553630-31
GAGCACA524700.023.7003549
ATGTCAG457150.023.47450834-35
Produced by FastQC (version 0.11.2)