FastQCFastQC Report
Mon 29 Jun 2015
G85_M7_ATGTCA_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M7_ATGTCA_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26523692
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC782380.29497401794591793Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT230750.039.539189
CGCCGTA153400.038.69400846-47
AAGAGCG261000.035.7054828
AGATCGG267800.034.5898861
GTCGCCG176150.033.63063444-45
CCGTATC177700.033.5989648-49
TCGTGTA174850.032.9770114-15
GATCTCG197700.032.77556634-35
GATCGGA298100.031.1370832
ATCGGAA312850.029.7136523
CGTATCA152900.029.24085450-51
CGGAAGA331650.028.8290185
TCTCGGT200700.028.8219236-37
TCGCCGT153550.028.39028446-47
CGTCGTG211600.027.67837712-13
TAGATCT228200.026.86665332-33
GCCGTAT167350.026.82716848-49
AGCGTCG232650.026.72352810-11
TCGGTGG227250.026.54931838-39
GTATCAT227200.026.15596650-51
Produced by FastQC (version 0.11.2)