FastQCFastQC Report
Mon 29 Jun 2015
G85_M8_GTCCGC_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M8_GTCCGC_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23996329
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC662480.2760755613910778Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT166650.044.1155439
AAGAGCG184050.040.1478428
CGCCGTA103750.038.99247746-47
GATCGGA211850.035.2965772
AGATCGG212300.035.0454371
CGTATCA105800.034.0836350-51
ATCGGAA226050.033.079323
TCGTGTA122750.032.97633714-15
GTCGCCG126550.032.27968644-45
TCGCCGT110650.031.93830546-47
CCGTATC128150.031.8403848-49
GCCGTAT114150.031.77374548-49
CGGAAGA235450.031.6802025
GATCTCG140100.030.55169934-35
GTCGTGT125550.029.0927414-15
CGTCGTG148850.027.53795812-13
TCGGAAG275650.027.346854
TCTCGGT153000.026.30421436-37
AGCGTCG163550.025.85919210-11
TAGATCT162550.025.80294632-33
Produced by FastQC (version 0.11.2)